Protein Extraction Kit

Protein Extraction Kits are specialized tools designed to isolate proteins from various biological samples such as tissues, cells, or body fluids. These kits simplify the process of breaking down cellular components to release proteins, ensuring consistency, reproducibility, and minimal degradation.

Content of Protein Extraction Kits

  • Lysis Buffer Solutions:
    • Detergents: Typically include non-ionic (e.g., NP-40, Triton X-100) or ionic detergents (e.g., SDS) to disrupt cell membranes and solubilize proteins.
    • Salts and Buffers: Buffers such as Tris-HCl, phosphate-buffered saline (PBS), or HEPES to maintain optimal pH and ionic strength.
  • Clarification Components:
    • Centrifuge Tubes and Filters: Designed to remove cell debris after lysis.
    • Solid-Phase Materials (if applicable): Some kits include affinity beads or chromatography columns for isolating specific protein subsets or purifying the extract further.
  • Sample Handling Accessories:
    • Pre-treated Vials and Microcentrifuge Tubes: To prevent protein adsorption and contamination.
    • Detailed Protocol Manual: Step-by-step instructions that include reagent preparation, incubation times, and temperature conditions.

Applications of Protein Extraction Kits

  • Proteomics Studies:
    • Extracted proteins can be analyzed using mass spectrometry, 2D gel electrophoresis, or liquid chromatography to profile the protein composition of cells or tissues, aiding in biomarker discovery or disease research.
  • Western Blotting and ELISA:
    • Purified proteins serve as samples for immunoassays to detect specific proteins, assess expression levels, or study protein interactions.
  • Enzyme Activity Assays:
    • Functional studies require active proteins from extracts to measure enzyme kinetics, substrate specificity, or inhibition by drugs.
  • Structural Biology:
    • Purified proteins obtained from these kits can be used in crystallography, NMR, or cryo-electron microscopy to determine protein structures.

Protein Extraction Kits offer a streamlined, standardized method to isolate proteins from complex biological matrices. The technical steps, including lysis, centrifugation, and optional purification, are critical for obtaining high-quality protein samples that retain their native or functional state.

689.38 689.38 USD
689.38 689.38 USD
689.38 689.38 USD
689.38 689.38 USD
689.38 689.38 USD
689.38 689.38 USD
689.38 689.38 USD
610.63 610.63 USD
869.38 869.38 USD
873.88 873.88 USD
689.38 689.38 USD
689.38 689.38 USD
610.63 610.63 USD
486.88 486.88 USD
706.25 706.25 USD
1,026.88 1026.88 USD
610.63 610.63 USD
610.63 610.63 USD
610.63 610.63 USD
873.88 873.88 USD
873.88 873.88 USD