Cytoplasmic & Nucléal DNA Extraction 

Cytoplasmic and Nuclear DNA extraction involves isolating DNA from distinct cellular compartments—cytoplasm and nucleus—enabling researchers to study different aspects of genetic material, such as mitochondrial DNA (predominantly found in the cytoplasm) and nuclear genomic DNA. Specialized kits or protocols are designed to separate these compartments efficiently, using differential lysis and centrifugation techniques.

Content of a Typical Cytoplasmic & Nuclear DNA Extraction Kit

  • Fractionation Reagents:
    • Differential Centrifugation Tools: Conical tubes or microcentrifuge tubes designed for separation steps.
  • Purification Components:
    • Silica Membrane Columns or Magnetic Beads: For binding and washing DNA during the purification process.
  • Sample Handling Accessories:

    • Detailed Protocol Manual: Step-by-step instructions for fractionation, lysis, centrifugation, and purification with safety and timing guidelines

Applications of Cytoplasmic & Nuclear DNA Extraction

  • Comparative Genomic Studies:
    • Analyze differences between mitochondrial (cytoplasmic) and nuclear genomes, including variations in sequence, copy number, or epigenetic modifications.
  • Disease Research:
    • Study mutations in mitochondrial DNA linked to metabolic disorders, neurodegenerative diseases, or aging, alongside nuclear DNA alterations.
  • Gene Expression and Regulation:
    • Investigate nuclear DNA modifications (e.g., methylation patterns) that affect gene expression, while separately assessing mitochondrial DNA for its role in cellular energy metabolism.
  • Forensic and Evolutionary Biology:
    • Extract mitochondrial DNA from the cytoplasm for forensic identification or phylogenetic studies, as mitochondrial DNA is more abundant and maternally inherited.

Cytoplasmic and Nuclear DNA extraction protocols are essential for isolating distinct genomic fractions from cells, enabling detailed compartment-specific genetic studies. Using tailored buffers, controlled lysis, and precise centrifugation, researchers can obtain high-purity DNA from each cellular compartment.

475.00 475.0 USD
475.00 475.0 USD