AffiEXTRACT® Single Nuclei Isolation Kit for Plant Tissues

The "AffiEXTRACT® Single Nuclei Isolation Kit for Plant Tissues" is designed to isolate individual nuclei from plant tissues, making it ideal for applications like single-nucleus RNA sequencing (snRNA-seq). This kit is specifically optimized for the unique challenges of working with plant tissues. Here’s how it works:

Step-by-Step Process:

1. Sample Preparation:

A small sample of plant tissue is collected and prepared for processing. Plant cells are often surrounded by tough cell walls, making it harder to isolate whole cells. This kit helps researchers overcome that by isolating nuclei directly from the tissue.

2. Tissue Dissociation:

The plant tissue is gently broken down into smaller pieces. This step is essential for releasing the nuclei from the plant cells. The dissociation process is carefully optimized to handle the tough structure of plant cells while preserving the nuclei for high-quality analysis.

3. Nuclei Isolation:

The kit’s specialized reagents help break open the plant cell membranes without damaging the nuclear membranes. This ensures that the nuclei are separated cleanly from the rest of the plant material, preserving them for analysis.

4. Nuclei Purification:

Once the nuclei are isolated, they are purified to remove any remaining cell debris, cytoplasmic material, or other unwanted components. This step ensures the nuclei are clean and intact for high-quality downstream applications.

5. Quality Check:

The isolated nuclei are then counted and assessed for quality. A microscope or automated counting device is often used to ensure that the nuclei are intact and suitable for further analysis, such as sequencing.

6. Downstream Applications:

The purified nuclei can be used for various downstream applications, including:

• Single-nucleus RNA sequencing (snRNA-seq): Particularly useful for plant tissues where isolating whole cells is difficult.
• Genomic studies: Researchers can use these nuclei to study gene expression and other genetic elements at the single-cell level.

Why Use This Kit for Plant Tissues?

• Optimized for Plant Cells: The kit is specifically designed to handle the tough cell walls and unique challenges of plant tissues.
• High-Quality Nuclei: It isolates pure, intact nuclei from plant tissues, ensuring reliable results in downstream applications.
• Enables Single-Nucleus Analysis: Especially important in plants where isolating whole cells is difficult, this kit provides access to the nuclear content for detailed genetic analysis.

Applications in Plant Research:

Using the isolated nuclei, researchers can:

• Study gene expression in plant tissues by focusing on the nuclear RNA.
• Explore cellular diversity in plants by examining different cell types and their genetic activity.
• Investigate plant diseases, development, or stress responses at the single-cell level by analyzing individual nuclei.

In summary, the AffiEXTRACT® Single Nuclei Isolation Kit for Plant Tissues offers a specialized solution for isolating high-quality nuclei from plant cells. It enables researchers to overcome the challenges of working with plant tissues and perform detailed genetic studies, including single-nucleus RNA sequencing.