AffiEXTRACT® Nuclei and Cytosol Isolation Kit for Adipose Tissues
CAT# AFG-INV-26
Size: 20 Tests
Storage Temperature (⁰C)
4⁰C
Shipping Temperature (⁰C)
Ambient
The "AffiEXTRACT® Nuclei and Cytosol Isolation Kit for Adipose Tissues" is designed to efficiently isolate both nuclei and cytosol from adipose (fat) tissue samples. This is especially useful for downstream applications like RNA or protein analysis. Here's how the kit works when used with adipose tissue:
Step-by-Step Process:
1. Sample Preparation:
A small sample of adipose tissue is collected for processing. Since fat tissues are often challenging to work with due to their structure, isolating nuclei and cytosol separately allows for more targeted analysis.
2. Tissue Dissociation:
The tissue is gently broken down into smaller pieces. This step is crucial for releasing the nuclei without damaging them. The kit also helps separate the cytosol from the tissue, ensuring both components are ready for analysis.
3. Nuclei and Cytosol Isolation:
Special reagents in the kit help break the cell membranes while preserving the nuclear membranes. This allows the nuclei to be separated from the cytosol. At the same time, the cytosol is also isolated, providing clean fractions of both components.
4. Purification:
The nuclei and cytosol are purified to remove any remaining debris or unwanted material. This ensures both the nuclear and cytosolic fractions are clean and of high quality, ready for further study.
5. Quality Check:
The number and quality of the isolated nuclei are assessed. A microscope or automated counting device may be used to ensure the nuclei are intact, and that both the nuclear and cytosolic fractions are suitable for downstream applications.
6. Downstream Applications:
Once isolated, the nuclei and cytosol can be used for various applications. For example:
- The nuclei can be used in single-nucleus RNA sequencing (snRNA-seq) or other genomic studies.
- The cytosol can be used for proteomic analysis or biochemical studies.
Why Use This Kit for Adipose Tissue?
- Effective for Fat Tissues: Adipose tissue is difficult to process because of its structure. This kit is specially designed to handle the unique challenges of fat tissue, making it easier to isolate both nuclei and cytosol.
- Dual Isolation: The kit isolates both nuclei and cytosol, providing researchers with two distinct fractions for more comprehensive studies.
- High Purity: The kit ensures that both the nuclear and cytosolic fractions are pure and free from contaminants, making them ideal for high-quality analysis.
Applications in Adipose Tissue Research:
Using the isolated nuclei and cytosol, researchers can:
- Study gene expression in adipose tissue by analyzing the nuclei.
- Investigate protein content and other cytosolic components for metabolic or biochemical studies.
- Explore the roles of different cell types in fat tissue and how they contribute to health or disease.
In summary, the kit provides an efficient method to isolate high-quality nuclei and cytosol from adipose tissue. This dual isolation approach allows researchers to perform in-depth studies on both the nuclear and cytosolic components of fat cells, opening doors to new insights in metabolic research, obesity, and related conditions.