AffiEXTRACT® Single Nuclei Isolation Kit for Cells & Tissues

The "AffiEXTRACT® Single Nuclei Isolation Kit for Cells & Tissues" is designed to isolate individual nuclei from various types of cells and tissues, making it ideal for downstream applications such as single-nucleus RNA sequencing (snRNA-seq). Here's how the kit works:

Step-by-Step Process:

1. Sample Preparation:

A small sample of tissue or a collection of cells is prepared for processing. This kit is suitable for a wide range of tissues and cell types, allowing you to isolate nuclei even from challenging samples.

2. Tissue and Cell Dissociation:

The tissue or cell sample is gently broken down into smaller parts. This step is crucial for releasing the nuclei from the sample while keeping them intact. The dissociation process is carefully designed to preserve the integrity of the nuclei for high-quality analysis.

3. Nuclei Isolation:

The kit uses specialized reagents that help break open the cell membranes while leaving the nuclei intact. This ensures that the nuclei are separated cleanly from the rest of the cell material, ready for further study.

4. Nuclei Purification:

After isolation, the nuclei are purified to remove debris and other unwanted components from the sample. The kit ensures that the nuclei are clean and intact, making them suitable for high-quality downstream applications.

5. Quality Check:

The number and quality of the isolated nuclei are checked. Researchers can use a microscope or automated counting device to ensure the nuclei are intact and suitable for further use in sequencing or other applications.

6. Downstream Applications:

Once isolated, these nuclei are perfect for various applications, including:

• Single-nucleus RNA sequencing (snRNA-seq): Ideal for tissues where isolating whole cells is difficult.
• Genomic and proteomic studies: Researchers can perform detailed analyses of the isolated nuclei to study gene expression and protein content.

Why Use This Kit for Cells and Tissues?

• Versatile: This kit works well with a wide range of cells and tissues, making it suitable for many types of research.
• High-Quality Nuclei: The kit ensures that the isolated nuclei are intact and pure, which is critical for accurate downstream analysis.
• Ideal for Difficult Samples: In cases where isolating whole cells is challenging, this kit allows researchers to focus on nuclei, providing a more feasible approach.

Applications in Research:

Using the isolated nuclei, researchers can:

• Analyze gene expression in difficult-to-process tissues using single-nucleus RNA sequencing.
• Explore cellular diversity by studying different cell types within a tissue, focusing on nuclear components.
• Study specific diseases like cancer, neurological disorders, or metabolic diseases by examining the genetic material inside individual nuclei.

In summary, the AffiEXTRACT® Single Nuclei Isolation Kit for Cells & Tissues provides a reliable way to isolate high-quality nuclei from a variety of samples. This makes it an essential tool for researchers who need to perform detailed genetic or cellular analysis, particularly in challenging tissues.