AffiEXTRACT® Gentle Single Nuclei Isolation Kit

The "AffiEXTRACT® Gentle Single Nuclei Isolation Kit - Detergent-Free" is designed to isolate intact nuclei without the use of harsh detergents, making it ideal for preserving sensitive cellular structures for downstream analysis. This kit is particularly useful when studying delicate tissues or processes where detergents may interfere. 

Step-by-Step Process:

1. Sample Preparation:

A small sample of tissue or cells is collected for processing. This kit is suitable for a wide range of sample types, including delicate tissues where using detergents could compromise the integrity of the nuclei.

2. Tissue or Cell Dissociation:

The tissue or cell sample is gently broken down into smaller pieces. This process avoids the use of harsh detergents, which can disrupt sensitive structures, ensuring that the nuclei remain intact during isolation.

3. Nuclei Isolation:

Specialized reagents in the kit allow for the gentle release of nuclei from the cells without the use of detergents. This process preserves the nuclear membrane, making sure the nuclei remain intact and ready for further analysis.

4. Nuclei Purification:

Once isolated, the nuclei are purified to remove debris and any other unwanted material. The purification process ensures that the nuclei are of high quality, free from contaminants that could affect downstream studies.

5. Quality Check:

The isolated nuclei are then counted and checked for quality. A microscope or an automated device can be used to confirm that the nuclei are intact and suitable for applications like RNA sequencing or genomic analysis.

6. Downstream Applications:

The detergent-free isolation process preserves the integrity of the nuclei, making them ideal for sensitive applications such as:

• Single-nucleus RNA sequencing (snRNA-seq): Preserves the nuclear RNA for detailed gene expression studies.
• Proteomic and genomic studies: Suitable for studying nuclear proteins and DNA.

Why Use a Detergent-Free Kit?

• Preserves Nuclear Integrity: This kit avoids using harsh chemicals, ensuring the nuclei remain intact for high-quality downstream analysis.
• Gentle on Delicate Tissues: It is particularly beneficial for tissues or cells that are sensitive to detergents, making it versatile for various research needs.
• Suitable for Sensitive Applications: Ideal for studies where the use of detergents could interfere with the analysis of nuclear components, such as in proteomics or RNA sequencing.

Applications in Research:

Using this kit, researchers can:

• Perform high-quality RNA sequencing by preserving the nuclear RNA.
• Study nuclear proteins in greater detail, without interference from detergents.
• Explore gene expression and other nuclear processes in sensitive tissues or cells.

In summary, the AffiEXTRACT® Detergent-Free Nuclei Isolation Kit offers a gentle yet effective way to isolate high-quality nuclei, making it perfect for sensitive applications where maintaining nuclear integrity is critical. It allows for accurate and detailed analysis without the risk of detergent-related interference.